GCP5 Antibody (E-1): sc-365837
- GCP5 Antibody (E-1) is a mouse monoclonal IgG2b κ GCP5 antibody, cited in 11 publications, provided at 200 µg/ml
- raised against amino acids 41-340 mapping near the N-terminus of GCP5 of human origin
- GCP5 Antibody (E-1) is recommended for detection of GCP5 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-GCP5 Antibody (E-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GCP5 Antibody (E-1) for WB applications. These reagents are now offered in bundles with GCP5 Antibody (E-1) (see ordering information below).
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GCP5 Antibody (E-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects GCP5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GCP5 Antibody (E-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GCP5, also known as gamma-tubulin complex component 5, is a crucial 1024 amino acid protein that plays a significant role in the γ-Tubulin complex, which is essential for microtubule organization and nucleation. This complex is primarily localized to the centrosome, where GCP5 facilitates the formation of microtubules, and to the cytoplasm, where GCP5 exists as soluble complexes that can be recruited to the centrosome as needed. Proper functioning of the γ-Tubulin complex is vital for cellular processes such as mitosis and intracellular transport, and defects in GCP5 have been linked to Prader-Willi syndrome, a genetic disorder characterized by obesity, behavioral issues, and cognitive impairments. The distinct roles of GCP proteins, including GCP5, underscore their importance in maintaining cellular integrity and function, making anti-GCP5 antibody (E-1) an invaluable tool for researchers studying microtubule dynamics and related pathologies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GCP5 Antibody (E-1) References:
- Human 76p: A new member of the gamma-tubulin-associated protein family. | Fava, F., et al. 1999. J Cell Biol. 147: 857-68. PMID: 10562286
- GCP5 and GCP6: two new members of the human gamma-tubulin complex. | Murphy, SM., et al. 2001. Mol Biol Cell. 12: 3340-52. PMID: 11694571
- Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. | Rush, J., et al. 2005. Nat Biotechnol. 23: 94-101. PMID: 15592455
- Microtubule nucleation and anchoring at the centrosome are independent processes linked by ninein function. | Delgehyr, N., et al. 2005. J Cell Sci. 118: 1565-75. PMID: 15784680
- GCP-WD is a gamma-tubulin targeting factor required for centrosomal and chromatin-mediated microtubule nucleation. | Lüders, J., et al. 2006. Nat Cell Biol. 8: 137-47. PMID: 16378099
- Donor centrosome regulation of initial spindle formation in mouse somatic cell nuclear transfer: roles of gamma-tubulin and nuclear mitotic apparatus protein 1. | Van Thuan, N., et al. 2006. Biol Reprod. 74: 777-87. PMID: 16407502
- PhLP3 modulates CCT-mediated actin and tubulin folding via ternary complexes with substrates. | Stirling, PC., et al. 2006. J Biol Chem. 281: 7012-21. PMID: 16415341
- Fluorescence resonance energy transfer and molecular modeling studies on 4',6-diamidino-2-phenylindole (DAPI) complexes with tubulin. | Arbildua, JJ., et al. 2006. Protein Sci. 15: 410-9. PMID: 16452620
- The mammalian gamma-tubulin complex contains homologues of the yeast spindle pole body components spc97p and spc98p. | Murphy, SM., et al. 1998. J Cell Biol. 141: 663-74. PMID: 9566967
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GCP5 Antibody (E-1) | sc-365837 | 200 µg/ml | $316.00 | |||
GCP5 Antibody (E-1): m-IgG Fc BP-HRP Bundle | sc-529486 | 200 µg Ab; 10 µg BP | $354.00 | |||
GCP5 Antibody (E-1): m-IgGκ BP-HRP Bundle | sc-522388 | 200 µg Ab, 40 µg BP | $354.00 | |||
GCP5 Antibody (E-1) AC | sc-365837 AC | 500 µg/ml, 25% agarose | $416.00 | |||
GCP5 Antibody (E-1) HRP | sc-365837 HRP | 200 µg/ml | $316.00 | |||
GCP5 Antibody (E-1) FITC | sc-365837 FITC | 200 µg/ml | $330.00 | |||
GCP5 Antibody (E-1) PE | sc-365837 PE | 200 µg/ml | $343.00 | |||
GCP5 Antibody (E-1) Alexa Fluor® 488 | sc-365837 AF488 | 200 µg/ml | $357.00 | |||
GCP5 Antibody (E-1) Alexa Fluor® 546 | sc-365837 AF546 | 200 µg/ml | $357.00 | |||
GCP5 Antibody (E-1) Alexa Fluor® 594 | sc-365837 AF594 | 200 µg/ml | $357.00 | |||
GCP5 Antibody (E-1) Alexa Fluor® 647 | sc-365837 AF647 | 200 µg/ml | $357.00 | |||
GCP5 Antibody (E-1) Alexa Fluor® 680 | sc-365837 AF680 | 200 µg/ml | $357.00 | |||
GCP5 Antibody (E-1) Alexa Fluor® 790 | sc-365837 AF790 | 200 µg/ml | $357.00 |